Module Catalogues, Xi'an Jiaotong-Liverpool University   
 
Module Code: BIO312
Module Title: Protein Structure and Function
Module Level: Level 3
Module Credits: 5.00
Academic Year: 2018/19
Semester: SEM2
Originating Department: Biological Sciences
Pre-requisites: N/A
   
Aims
Proteomics is the term used to describe the detailed characterisation of proteins within cells and tissues, and this module begins by describing the latest proteomic technologies. It then gives some examples of how these proteomic approaches can solve both fundamental and applied problems across a range of biological disciplines, including vaccine development. The second half of the module introduces cutting edge technologies capable of solving detailed, fine structures of proteins, and describes how the knowledge of protein structure can help explain the function of specific systems such as cellular energy production, ion channels and receptor signalling.
Learning outcomes 
A. Understand and compare the latest technologies used for extraction, enrichment and analysis of proteins. A critical commentary on the pros and cons of the methods and their limitations for proteins of different characteristics. Design and create, with justification, a protocol to purify a protein from a natural source using knowledge learnt in the lectures.

B. Discuss how proteomics-based approaches can be used to study fundamental and applied biological problems, such as vaccine development.


C. Analyse the different methods of analysis for post-translational modifications of proteins and their implications for cell function. Compare and contrast the different methods using specific examples from the literature.

D. Evaluate the strength and weaknesses of NMR, x-ray crystallography, surface plasmon resonance and other physical methods that can be used to determine the detailed structure of proteins using specific examples from the literature. Draw connection among different techniques as a way to realise their potentials in protein characterisation.

E. Appraise and discuss how knowledge of protein structure can be used to explain function, in particular the structural basis of receptor signalling and the function of molecular 'motors' such as ATP synthase.


Method of teaching and learning 
Key topics will be introduced through standard lectures, supported by materials on VITAL and other web-based resources. Additionally, practical demonstrations and workshops will aid learning. Students will be guided to key articles in the literature (original papers and review articles) and be expected to use this material to supplement their independent learning. A key principle of this module will be both the understanding and application of techniques in proteomics research, and at regular intervals both formative and summative assessment exercises will test knowledge and ability to solve problems.
Syllabus 
Methods of Proteome Analysis. (Lecture 1-4);

Applications of Proteomic Techniques ( Lecture 5-6);

Applications of Proteomic Techniques II. (lecture 7-8);

Acquisition of protein structure (lecture 9-10);

Methods of structural Determination (lecture 11-12);

Nuclear magnetic resonance (Lecture 13-14);

Proteins in Action: Relating Structure to Function (Lecture 17-18);


Lecture 1 : Revision protein structure and methods of analysis


Lecture 2 : Microcharacterisation of proteins


Lecture 3 : Identification of proteins in complex mixtures


Lecture 4 : Advanced proteomic techniques


Lecture 5: Subcellular proteomics


Lecture 6: Post-translational modifications


Lecture 7: Proteomics in microbial pathogenesis


Lecture 8: Vaccine development


Lecture 9: Protein folding in vivo and in vitro


Lecture 10: Protein folding in vivo and in vitro


Lecture 11: Overview of techniques and applications


Lecture 12: X-ray crystallography


Lecture 13: Nuclear magnetic resonance: Structural Determination


Lecture 14: Nuclear magnetic resonance: Molecular Dynamics & Interactions


Lecture 15: Alternative methods of structural analysis: Circular dichroism; electron microscopy; fluorescence


Lecture 16: Modelling: Computational Prediction of Structure & Dynamics


Lecture 17: Structural basis of receptor signalling


Lecture 18: Structural basis of receptor signalling


Lecture 19: Molecular machines and motors 1: Receptors and channels


Lecture 20: Molecular machines and motors 2: ATP synthase
Delivery Hours  
Lectures Seminars Tutorials Lab/Prcaticals Fieldwork / Placement Other(Private study) Total
Hours/Semester 26     13      111  150 

Assessment

Sequence Method % of Final Mark
1 Final Exam 75.00
2 Coursework Assignment 1 12.50
3 Coursework Assignment 2 12.50

Module Catalogue generated from SITS CUT-OFF: 4/20/2018 8:42:23 AM